Flow cytometry and other single cell technologies 

Extensive experience with flow cytometry analysis and sorting of human and mouse lung, skin, hair follicle and thymus. I learned FACS during my PhD in the US, at University of Pittsburgh and at Duke University.  Flow cytometry was the entry point into the single cell world. Since then, I implemented single cell technologies both at EPFL (a Biomark, Fluidigm instrument) as well as in Giessen. In Giessen, we purchased several single cell instruments, we trained users and provided opportunities for continual training in the form of workshops, and single cell clubs. 

Human alveolar epithelial type II cells in healthy and IPF lung

What is an alveolar epithelial cells? Are there subtypes of AEC2s in the human lung? How are they replenished? And how does everything change in IPF. I started with flow cytometry characterization, based on known markers. Next I performed microarray transcriptomic analysis on sorted human AEC2s from normal and IPF lungs. I found new markers and identified new processes that improved our understanding of the human lung.

Roxana Wasnick MD PhD 

Medical Scientist

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